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Re-creating Alexander Fleming’s famous experiment that led to the discovery of the antibiotic penicillin, students intentionally contaiminate a steril broth culture with organisms from their own skin. They then grow and isolate bacteria from different colonies on sterile nutrient agar and place the colonies in a sterile broth to create a pure culture of each type of bacteria. After placing the pure cultures on sterile nutrient agar, students use a paper disk containing a known quantity of penicillin to test whether that particular bacteria is killed or is completely unaffected. Students gain an appreciation of the chance nature of Fleming’s discovery by noting that only some, but not all, bacteria are susceptible to the antibiotic penicillin. Developed by Michael Sulzinski. Includes materials for 18 student groups and instructions. Requires, but does not include penicillin discs. Grades 10–12.


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