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Teacher Developed Classroom Tested

Includes materials for five labs
Transformation, a basic principle of genetic engineering, is investigated in this lab.
Students take the bacteria E. coli JM 101, which is sensitive to ampicillin and incapable of producing beta-galactosidase needed for lactose breakdown, and transform it by inserting the pUB8 plasmid. The bacteria thus receives antibiotic resistance as well as lac+phenotype.
In two 45-minute labs the traits are transferred and the transformed cells are grown on agar plates containing the antibiotic and on plates with ampicillin and x-gal, a histochemical substrate for B-galactosidase which yields a blue precipitate upon hydrolysis. Transformations appear as white colonies on plates with ampicillin, and blue colonies on plates with ampicillin and x-gal.
Includes: materials for five labs (recommended six students each), teacher's guide, student study and analysis copymasters with background information, time requirements, instructions, data table for analysis, and study questions.
An incubator and microcentrifuge are required for this experiment
Note: Live materials (67498-01) are required but not included.
The live materials set Includes the reagent set, E. coli, and plasmid DNA. A ship date is required.

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