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Phenotype Expression Kit
Phenotype Expression Kit

Product NameSKUPriceQty
Kit, Phenotype Expression, w/o Perishables
WW6385801
 $37.00 
Perishable Material (Reagent Set, E. coli & Plasmid DNA) for Phenotype Expression Kit (63858-01) **SHIP DATE REQUIRED**
This item is restricted, it only ships to registered educational institutions.
WW6749801
 $66.25 




Product Information

Transformation, a basic principle of genetic engineering, is investigated in this lab. Students take the bacteria E. coli JM 101, which is sensitive to ampicillin and incapable of producing beta-galactosidase needed for lactose breakdown, and transform it by inserting the pUB8 plasmid. The bacteria thus receives antibiotic resistance as well as lac+phenotype. In two 45-minute labs the traits are transferred and the transformed cells are grown on agar plates containing the antibiotic and on plates with ampicillin and x-gal, a histochemical substrate for B-galactosidase which yields a blue precipitate upon hydrolysis. Transformations appear as white colonies on plates with ampicillin, and blue colonies on plates with ampicillin and x-gal. Includes teacher’s guide, student study and analysis copymasters with background information, time requirements, instructions, data table for analysis, and study questions. The live materials reagent set, available seperately, contains E. coli, and plasmid DNA. A ship date is required. An incubator and microcentrifuge are required for this experiment, but not included.
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